how to make several small DNA fragnent copy together
p.r.ashby at MAPS.dundee.ac.uk
Thu Nov 14 05:35:52 EST 2002
In article <20021114060159.22742.qmail at ww02.hostica.com>,
yujianzhang at yahoo.com wrote:
> Now I am trying to construct a expression vector containing several copies of
> small DNA fragments(150bp). These fragments should be joined by head-to-tail
> for correct translation. Does any one has a good solution?
Order complementary phosphorylated oligos with non complementary
overhangs. Heat and slowly anneal equimolar amounts of the oligos.
Ligate a range of insert:vector ratios. Transform and analyse numbers by
your method of choice (I used high% LMP agarose gels).
Wellcome Trust Biocentre
University of Dundee, Scotland
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