color of loading dye

Peter Ashby p.r.ashby at MAPS.dundee.ac.uk
Thu Nov 14 05:38:19 EST 2002


In article <3DD2E18A.BF712520 at uow.edu.au>,
 Mark Dowton <Mark_Dowton at uow.edu.au> wrote:

> It's definitely a pH problem.  We add around 40-50 ul of 1 M Tris per ml of
> dye to turn it from yellow to blue.  This will also increase the solubility of
> the dye.

for pcr I dissolve Orange G to saturation in 100% glycerol, for use I 
dilute 50/50 with TE and use as 10x. This runs at about 60bp and won't 
obscure your bands like bromophenol.

Peter

-- 
Peter Ashby
Wellcome Trust Biocentre
University of Dundee, Scotland
Reverse the Spam and remove to email me.



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