color of loading dye
p.r.ashby at MAPS.dundee.ac.uk
Thu Nov 14 05:38:19 EST 2002
In article <3DD2E18A.BF712520 at uow.edu.au>,
Mark Dowton <Mark_Dowton at uow.edu.au> wrote:
> It's definitely a pH problem. We add around 40-50 ul of 1 M Tris per ml of
> dye to turn it from yellow to blue. This will also increase the solubility of
> the dye.
for pcr I dissolve Orange G to saturation in 100% glycerol, for use I
dilute 50/50 with TE and use as 10x. This runs at about 60bp and won't
obscure your bands like bromophenol.
Wellcome Trust Biocentre
University of Dundee, Scotland
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