color of loading dye

Peter Ashby p.r.ashby at
Thu Nov 14 05:38:19 EST 2002

In article <3DD2E18A.BF712520 at>,
 Mark Dowton <Mark_Dowton at> wrote:

> It's definitely a pH problem.  We add around 40-50 ul of 1 M Tris per ml of
> dye to turn it from yellow to blue.  This will also increase the solubility of
> the dye.

for pcr I dissolve Orange G to saturation in 100% glycerol, for use I 
dilute 50/50 with TE and use as 10x. This runs at about 60bp and won't 
obscure your bands like bromophenol.


Peter Ashby
Wellcome Trust Biocentre
University of Dundee, Scotland
Reverse the Spam and remove to email me.

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