cloning problem

nazarian80 at yahoo.com nazarian80 at yahoo.com
Sun Nov 17 10:51:44 EST 2002


hi
I have attempted to clone a 1.3 kb insert (Bamh1/Xho I) into a PRSETA and PET28a vector (Bamh1/xho1)
  The vector has been gel purified and  fragments ligated (NEB T4 ligase overnight at 14 C),
and transformed into BL21 DE3 cells .   After
plating/overnight incubation, very few colonies are present OR not found, and after screening
for positives with Bamh1/Xho I, there is no insert.  Any suggestion/tips?


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