lambda DNA

Carnac carnac at
Mon Nov 18 23:42:14 EST 2002

"Ong" <boch19 at> wrote in message
news:arc4n7$aa8$1 at
> the size of band is around 23kb. How can I ensure that is the DNA or RNA.
> Thank for your help.

If you've only got a single band on the extract gel, it most certainly
wouldn't be RNA... Use a control to make sure your enzymes are functional.
Cut up your extracted lambda as well as the original lambda DNA.

23kb seems small for a viable phage, but I have heard of viable phages being
produced from smaller DNA.  Were your phage viable?

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