carnac at realitys-edge.com
Mon Nov 18 23:42:14 EST 2002
"Ong" <boch19 at pd.jaring.my> wrote in message
news:arc4n7$aa8$1 at news6.jaring.my...
> the size of band is around 23kb. How can I ensure that is the DNA or RNA.
> Thank for your help.
If you've only got a single band on the extract gel, it most certainly
wouldn't be RNA... Use a control to make sure your enzymes are functional.
Cut up your extracted lambda as well as the original lambda DNA.
23kb seems small for a viable phage, but I have heard of viable phages being
produced from smaller DNA. Were your phage viable?
More information about the Methods