protein precipitation

get real allen at NOSPAM.icm.umontreal.ca
Wed Nov 20 11:10:22 EST 2002


On Wed, 20 Nov 2002 8:54:24 -0500, s.hannier at abdn.ac.uk wrote
(in message <20021120135424.4318.qmail at ww02.hostica.com>):

> 
> 
> What is the best method to precipitate protein in a sample of less than 1 ug 
> in 1 ml What is the recovery ?
> 
> Thanks,
> 
> Sigrid
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&beg
> in=0

You may want to try concentration with a spin concentrator (eg Amicon's 
centricon). Precipitation techniques reduce the solubility of the protein. 
The recovery is nover 100% and will vary with each protein and with the 
technique you employ. However, there will likely be some loss in the 
centricon due to proein binding to the surfaces of the chamber and the 
filtration membrane.




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