fixation with FITC and PI

Manu Kohli mkohli at jhmi.edu
Wed Nov 20 14:10:40 EST 2002


Does anyone know whether formaldehyde fixation of cells will quench
the fluorescence of FITC and PI?  In other words, if I keep the cells
suspended in formaldehyde, will the dyes still fluoresce?  What about
in methanol?

Thanks,

Manu Kohli



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