Emergent-----Cloning problem

Haojiang Luanhaojiang at hotmail.com
Thu Nov 21 15:05:28 EST 2002


Hi, Dear all.

I am making a construct for mice knockout. After the ligation of the
insert and the vector and transfect into stable II cell, I got plenty
of colonies. But when I screen the colonies, most of them is smaller
in size than the vector! I had tried this for quite a few times, but I
can not get it. Anyone please give me some hints?!!!!

Thanks!



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