Wiil Glycerine, formaline, create problems for PCR

dr_pandu at yahoo.co.in dr_pandu at yahoo.co.in
Fri Nov 22 07:21:19 EST 2002


hubahopp at gmx.de (Wolfgang Schechinger) wrote in message news:<3.0.6.32.20021121163352.007cc2c0 at pop.gmx.net>...
> Dear Pandu, 
> 
> formaldehyde is likely to impair your polymerase activity. To my knowledge,
> HCHO is used in some hot start applications. The result largely might
> depend on final concentrations and the individual reaction conditions, also
> the presence of HCHO alternative targets like albumin.
> Glycerol will affect stringency. To be sure, better precipitate and wash
> your DNA (your virus should be inactivated) or at least use a PCR buffer
> that contains ammonium sulfate (might scavenge some HCHO).
> 
> Or just try and make some quality control.
> 
> Regards,
> 
> Wolfgang
> 
> 
> At 07:16 AM 2002/11/21 -0800, dr_pandu at yahoo.co.in wrote:
> >Dear All,
> >
> >I preserved suspected material of BHV-2 Virus in 50% glycerine PBS and
> >10% formaline.
> >
> >Let me know how to proceed further for conducting PCR?
> >
> >Eagerly waiting for the reply
> >
> >Yours
> >pandu
> >
> >
> 
> ---
Dear all,

What is the best way to preserve viral material for PCR?

I have to collct them from the field Veterinarians of India where
facilities for preservation are scant.

how many days can i preserve vesicular fluid containing virus at 4
degree centigade to use it for PCR
yours 
pandu



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