wanted: info on old Spinco rotor

Wolfgang Schechinger hubahopp at gmx.de
Sat Nov 23 16:34:06 EST 2002


Kyle, 

maybe you're lucky reconstructing the parameters from a publication that
used the rotor? Or have a look into a standard  book on ultracentrifugation
(like Ultracentrifugation, a practical approach from Oxford University
Press. Your library should stock it.)

Regards,

Wo

At 05:06 PM 2002/11/22 -0500, Kyle Legate wrote:
>
>Hi, I wonder if someone with some _old_ lab equipment can help me out. I'm
>looking for spec info on the Spinco SW-39 rotor. It holds ~5 ml/tube and
>I'm assuming it's functionally equivalent to Beckman's SW-55 rotor, but I
>want to make sure before I overspin/underspin all my samples. What I am
>trying to do is replicate some data from the early 70s by separating
>ribosomal subunits from intact ribosomes on a sucrose gradient. I have the
>assay working beautifully in a SW-28 but I'm looking to drastically scale
>down. A 3 hour spin vs a 16 hour spin is also very appealing. The paper I
>have gives rpm and time for the SW-39 and I'd like to adapt it to the
>SW-55. Unfortunately, the Beckman site doesn't have a historical records
>section!
>	Thanks for any help.
>
>... . . .  .  .  .    .    .    .     .     .     .      .      .      .
>legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com
>
>   Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
>    moon musick:ritual:IDM:experimental(electronica):minimalism:glitch
>.      .      .     .     .     .    .    .    .   .   .   .  .  . . ...
>
>

---



More information about the Methods mailing list