any hints for a plant mRNA extraction/purification kit?
Bryan J. Maloney
cavaggione at sbcglobal.net.spam.must.die.com
Mon Nov 25 22:51:54 EST 2002
"L. Lintott" <llintott at shaw.ca> wrote in news:3DD32628.A5E084E5 at shaw.ca:
> I don't know much about the mRNA purification part, but Trizol works
> well for extracting RNA from plants. I have used it for both potato and
> wheat, just make sure you do the extra steps for high carbohydrate
> material as recommended by LifeTechnologies (aka Invitrogen now).
You could follow up the Trizol extraction with poly-A bead treatment.
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