semi-quantitative RT-PCR

Stefi~ xxxgifalone at tin.it
Wed Nov 27 14:13:14 EST 2002


Hi everyone,
I need to relative quantify the amount of a mRNA during embryonic and
metamorphic development in the common toad.
I've recently bought a kit from Ambion: 18S Internal Standard, in which the
18S rRNA is co-amplified together with my mRNA of interest.
Is there anyone of you who has already used this kit? Any particular
suggestion? Tips and tricks?
Thanx so much in advance.

Stefano
xxxgifalone at tin.it (remove xxx)





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