Plasmid DNA from cloned CHO cells

caulfield caulfield_z at yahoo.com
Tue Oct 1 09:02:25 EST 2002


radio1_mike at yahoo.com wrote:
> Is this even possible? I have always had the assumption that pDNA was usually produced by bacterial culture. And I have done enough pDNA extractions from E. coli and VREs. Not familiar w/tissue culture...
> I have searched on the web, and all references I generate refer to gDNA extraction from mammalian (eg. CHO) cells and pDNA extraction from bacterial cultures.

I am not 100 percent sure of this but how about looking up the Hirt low 
molecular weight DNA extraction protocol. It is usually used to extract viral
genome from infected eukaryotic cells.




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