How to get a sufficent DNA from gel for ligation?

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Tue Oct 1 10:28:52 EST 2002


Call me frugal, but I'd stretch that 100 ng to be enough vector for 
5-10 (20 ul) ligation reactions!

Mike

>
>I use the Quiagen gel extraction kit. Usually about 100 ng of vector DNA is
>sufficient for one ligation reaction (20 microliters). The kit will give you a
>good yield depending on the starting amout of cut vector that you run on gel.

---



More information about the Methods mailing list