a problem in making the stable cell line of a suspension cell

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Oct 2 19:01:42 EST 2002


On 2 Oct 2002 15:20:45 -0000, linglingyang at yahoo.com wrote:

>Hi, guys. I have a problem in making the stable cell line of a suspension cell (U266). Despite of optimization by electroporation and other methods, its transfection efficiency is low. So how can I separate the transfected live cells from hundreds of dead cells around them? Only wait for the live cells to grow and out number the dead cells? The U266 grows pretty slow, though. What a headache!
>

I suppose you could cotransfect with a GFP plasmid and FACS sort them.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu



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