pGFLEX

sfrye at operamail.com sfrye at operamail.com
Thu Oct 3 03:39:13 EST 2002


Hi,

I found this on http://www.atcc.org/Products/vectors.cfm


LOCUS       pGFLEX       6143 bp dsDNA    Circular
DEFINITION  Mammalian cell expression vector with GST epitope tag
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     http://www.informaxinc.com/view/viewer.html
COMMENT     VNTREPLTYPE|Phagemid|
COMMENT     VNTEXTCHREPL|Bacteria|
COMMENT     VNTKW|ATCC 87629|
COMMENT     VNTKW|CMV promoter|
COMMENT     VNTKW|GST fusion protein|
COMMENT     VNTKW|G418R|
FEATURES               Location/Qualifiers
     promoter        1..686
                     /label=CMV
     promoter        687..847
                     /label=lac\promoter
     misc_feature    848..886
                     /label=beta-gal\leader\sequence
     CDS             887..1516
                     /label=GST\pi
     misc_binding    1517..1524
                     /label=thrombin\cleavage\site
     misc_feature    1570..1572
                     /label=TGA
     rep_origin      1895..2413
                     /label=f1\ori
     promoter        2466..2800
                     /label=SV40\promoter
     rep_origin      2669..2754
                     /label=SV40\ori
     CDS             2836..3630
                     /label=G418R
     polyA_signal    3804..3939
                     /label=SV40\polyA
     rep_origin      4373
                     /label=pMB1\ori
     CDS             complement(5131..5991)
                     /label=ampR
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
actatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttaa ctggcttatc
gaaattaata
      661 cgactcacta tagggagacc caagctctgg cacgacaggt ttcccgactg
gaaagcgggc
      721 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
ggctttacac
      781 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
tcacacagga
      841 aacagctatg accatgatta cgaattcatc gatgaattcc gccaccatgc
ccccctacac
      901 cgtggtctat ttcccacttc gaggccgctg cgcggccctg cgcatgctgc
tggcagatca
      961 gggccagagc tggaaggagg aggtggtgac cgtggagacg tggcaggagg
gctcactcaa
     1021 agcctcctgc ctatacgggc agctccccaa gttccaggac ggagacctca
ccctgtacca
     1081 gtccaatacc atcctgcgtc acctgggccg cacccttggg ctctatggga
aggaccagca
     1141 ggaggcagcc ctggtggaca tggtgaatga cggcgtggag gacctccgct
gcaaatacat
     1201 ctccctcatc tacaccaact atgaggcggg caaggatgac tatgtgaagg
cactgcccgg
     1261 gcaactgaag ccttttgaga ccctgctgtc ccagaaccag ggaggcaaga
ccttcattgt
     1321 gggagaccag atctccttcg ctgactacaa cctgctggac ttgctgctga
tccatgaggt
     1381 cctagcccct ggctgcctgg atgcgttccc cctgctctca gcatatgtgg
ggcgcctcag
     1441 cgcccggccc aagctcaagg ccttcctggc ctcccctgag tacgtgaacc
tccccatcaa
     1501 tggcaacggg aaacagctgg ttccgcgtgg atccccggaa ttcccgggtc
gactcgagcg
     1561 gccgcatcgt gactgactgg gccctattct atagtgtcac ctaaatgcta
gagctcgctg
     1621 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
cccccgtgcc
     1681 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
aggaaattgc
     1741 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
aggacagcaa
     1801 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
ctatggcttc
     1861 tgaggcggaa agaaccagct ggggctcgag gggggatccc cacgcgccct
gtagcggcgc
     1921 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
ccagcgccct
     1981 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
gctttccccg
     2041 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
ggcacctcga
     2101 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
gatagacggt
     2161 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
tccaaactgg
     2221 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
tggggatttc
     2281 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
ttaacaaaat
     2341 attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
ggcttttctg
     2401 attatcaacc ggggtggcga gctcgaattc tgtggaatgt gtgtcagtta
gggtgtggaa
     2461 agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa
ttagtcagca
     2521 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
catgcatctc
     2581 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
aactccgccc
     2641 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
agaggccgag
     2701 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
aggcctaggc
     2761 ttttgcaaaa agctcccggg agcttggata tccattttcg gatctgatca
agagacagga
     2821 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
ggccgcttgg
     2881 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
tgatgccgcc
     2941 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
cctgtccggt
     3001 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
gacgggcgtt
     3061 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
gctattgggc
     3121 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
agtatccatc
     3181 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
attcgaccac
     3241 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
tgtcgatcag
     3301 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
caggctcaag
     3361 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
cttgccgaat
     3421 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
gggtgtggcg
     3481 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
tggcggcgaa
     3541 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
gcgcatcgcc
     3601 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
atgaccgacc
     3661 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
tatgaaaggt
     3721 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
ggggatctca
     3781 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
tacaaataaa
     3841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
agttgtggtt
     3901 tgtccaaact catcaatgta tcttatcatg tctggatccc gtcgacctcg
agagcttggc
     3961 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
ttccacacaa
     4021 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
gctaactcac
     4081 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
gccagctgca
     4141 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
cttccgcttc
     4201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
cagctcactc
     4261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
acatgtgagc
     4321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
ttttccatag
     4381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
ggcgaaaccc
     4441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
gctctcctgt
     4501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
gcgtggcgct
     4561 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
ccaagctggg
     4621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
actatcgtct
     4681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
gtaacaggat
     4741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
ctaactacgg
     4801 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
ccttcggaaa
     4861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
gtttttttgt
     4921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
tgatcttttc
     4981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
tcatgagatt
     5041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
aatcaatcta
     5101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
aggcacctat
     5161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
tgtagataac
     5221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
gagacccacg
     5281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
agcgcagaag
     5341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
aagctagagt
     5401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
gcatcgtggt
     5461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
caaggcgagt
     5521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
cgatcgttgt
     5581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
ataattctct
     5641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
ccaagtcatt
     5701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
gggataatac
     5761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
cggggcgaaa
     5821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
gtgcacccaa
     5881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
caggaaggca
     5941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
tactcttcct
     6001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
acatatttga
     6061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
aagtgccacc
     6121 tgacgtcgac ggatcgggag atc
//

On 30 Sep 2002 14:40:11 +0100, abrown at nimr.mrc.ac.uk (Alex Brown)
wrote:

>Hello.
>
>I was wondering if anybody has the sequence for the vector pGFLEX.
>
>Thanks,
>
>Alex Brown.
>
>---

**************************
mailto:sfrye at operamail.com



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