Cant get rid of 2kb contaminant band during cloning
rpandy2000 at yahoo.com
rpandy2000 at yahoo.com
Fri Oct 4 16:27:36 EST 2002
clone has a 5kb insert in a 4kb vector. After mini/midi preps, however only a weak band at 9kb
observed.Major band is a 2kb one on 1% agarose gel. gel isolated 9kb band, transformed and
regrew up cultures...2kb band shows up again...restriction digests do not cut 2kb band but does
cut 9kb band. sequencing indicates presence of complete insert in the DNA prep...HELP
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0
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