Polymerase antibodies

D.K. dk at no.email.thankstospam.net
Sat Oct 5 12:39:25 EST 2002


Sven Even Finborud Borgos <svenb at chembio.ntnu.no> wrote:
>Some polymerases are sold as mixtures with antibodies that are supposed to
> reversibly inhibit the enzyme during setup and initial temperature ramping. Do
> any of you know whether these antibodies are sufficiently thermostable to work
> through all cycles, or are they permanently 
>denatured (=destroyed) during first denaturation of DNA?

All antibodies die very fast past +70C

DK



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