Please help with stable transfection

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Mon Oct 7 19:13:12 EST 2002


On 5 Oct 2002, John Ladasky wrote:

> The problem of making stable transfectants that are truly stable is
> unfortunately a common one.  Like you, I have had the percentage of
> fluorescence-positive cells decline as I continue to grow cells.
> Other researchers in the laboratories where I have worked have also
> had trouble.  It doesn't always happen, but when it does it is
> extremely frustrating.
>
Couldn't this arise from gene silencing? Although you have generated a
stable transfectant, the cells may have figured out a way to shut off the
gene of interest.

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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