cDNA library for EST project

Mark Cock cock at sb-roscoff.fr
Wed Oct 9 06:56:15 EST 2002


Hello,

I would like to construct cDNA libraries of the brown algae Ectocarpus 
for an EST project. Can anyone recommend a simple method that gives 
both a maximum of full length clones and cDNAs that are easy to 
sequence?

I have made libraries in the past using Clontech's SMART procedure but 
the strain used for plasmid rescue gave pour quality DNA in our hands, 
necessitating the sequencing of PCR products. What about the Superscipt 
kit, has anyone used this for an EST library?

Please send a copy of your reply to cock at sb-roscoff.fr.

Thanks, Mark

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