Gene Synthesis by overlap PCR

Duncan Clark junk at
Thu Oct 10 08:31:08 EST 2002

Hi Folks,

I intend to produce a 750 bp product with optimal codon usage using 
80-100mer oligos using minimal cycling proof-reading PCR. I've done 
450bp before with 50mers but need a bit longer this time.

In the past I have designed the oligos one by one using Oligo5. Now I 
can do the same for this gene but wonder if anyone knows of a, 
presumably, web based tool that will do all oligos automatically 
obviously minimising dimers etc etc

Many thanks

I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

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