Gene Synthesis by overlap PCR
junk at hgmp.mrc.ac.uk
Thu Oct 10 08:31:08 EST 2002
I intend to produce a 750 bp product with optimal codon usage using
80-100mer oligos using minimal cycling proof-reading PCR. I've done
450bp before with 50mers but need a bit longer this time.
In the past I have designed the oligos one by one using Oligo5. Now I
can do the same for this gene but wonder if anyone knows of a,
presumably, web based tool that will do all oligos automatically
obviously minimising dimers etc etc
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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