DNA stuck in wells!

Craig Lewis clewis at eclipse.net
Thu Oct 10 15:38:44 EST 2002


Has anyone experienced DNA from a simple restriction digest fail to move
in an agarose gel? This is a relatively recent problem I'm having. It
seems that the DNA gets stuck in the well. The gel is made with TAE
buffer and good quality agarose with EtBr added to the gel. The DNA is
in the 1 to 10 kb range and is quite clean. I'm using a
sucrose/bromophenol blue loading dye.

I can provide more details if needed.

Thanks,
Craig




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