DNA stuck in wells!

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Thu Oct 10 19:05:10 EST 2002


On Thu, 10 Oct 2002, Craig Lewis wrote:

> Has anyone experienced DNA from a simple restriction digest fail to move
> in an agarose gel? This is a relatively recent problem I'm having. It
> seems that the DNA gets stuck in the well. The gel is made with TAE
> buffer and good quality agarose with EtBr added to the gel. The DNA is
> in the 1 to 10 kb range and is quite clean. I'm using a
> sucrose/bromophenol blue loading dye.
>
Does the dye move?

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

   Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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