Sepharose bead electrophoresis

Prashant Srinivasan prashant at yngwie.com
Fri Oct 11 05:02:47 EST 2002


Hi all,

I'm trying to see bands of fluroscence on a chip electrophoresis
device.The sample to be used is FITC tagged antibody.The chip is made
of Si and the channels are oxide coated.The excitation setup consists
of a 488nm Ar-ion src. Detection is on a Color CCD.
A few questions:

1.Is there some kind of ch passivation necessary?
2.Would the use of Sepharose beads improve detection efficiency (by
descretizing the sample)?

Thanks in advance.

Prashant.



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