&replyto=ancaej$cofuo$3@ID-152646.news.dfncis.de&subject=Re: How to get a sufficent DNA from gel for ligation

Jon Rees jonnyr9 at r9corporation.fsnet.co.uk
Tue Oct 15 02:44:53 EST 2002


Daer Caulfield -

The APBiotech GFX kit is the best gel extraction (using a TAE running buffer
not borate) kit I have come across.  If you use hot water to elute - its
even better, and add a little SDS (0.5%) if you're eluting DIG-labelled
probes.  Its better than glass milk - and not too expensive!

best wishes

Dr Jonathan Rees
http://www.molbiol.net


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