stripping dig northerns

[jon at molbiol.net]

Dear RSPathis,

I use 2*SSC 0.1% SDS just off boiling (wrap a couple of paper towels around the bottle so you don't burn your hands!), and I do this twice with 100-200mL for 15-20 minutes.

I have most of my protocol for DIG down here:

http://www.r9corporation.fsnet.co.uk/Methods/dignorthernf.htm

If you want to discuss your protocol, or improvements you have made then you can also look here: http://www.bioinformatics.vg/cgi-bin/yabb/YaBB.pl

best wishes,

Dr Jon Rees



rspathis at binghamton.edu wrote:

> I am trying to strip northern blots probed with dig labeled cDNA and have been
> having some problems....does  anyone have a recipe and protocol?

> thanks


> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0





http://biowww.net/mynews/tree.php?group_name=bionet.molbio.methds-reagnts&begin=0