site-directed pcr mutagenesis

Peter Cherepanov peter.cherepanov at uz.kuleuven.ac.be
Thu Oct 17 15:09:09 EST 2002


1) which enzyme do you use for the final PCR? -This is very important. I
would recommend Expand. Or use Taq for the first few cycles.

2) double check your primers

3) if everything fails ... may be try to amplify a shorter piece and then
assamble the whole thing (by PCR or cloning)

hope this helps,

Peter

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