Problems with FLAG E.coli expression system from SIGMA

Trond Erik Vee Aune trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no
Fri Oct 18 01:55:36 EST 2002


Hi all,

Some at my lab are experiencing problems when using the FLAG E.coli 
expression system from SIGMA. After growing the cells they want to do a 
protein gel. For some reason they only get a smear of proteins, not able 
to see distinct bands. According to the protocol the cells are not 
supposed to be lysed. How can this be?

Regards,
Trond Erik




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