E. coli protein after NiNTA

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Oct 21 10:45:13 EST 2002


On Sun, 20 Oct 2002, Michael Witty wrote:

> > Yes, I also thought about His patches, but I don't remember seeing anywhere
> > that Ni can bind a discontinuous 6his epitope. Any references for that?
> > Dima's explanation sounds very plausible, too. As for Glucosamine-6-P
> > Synthase, it is possible that since it has a his-rich patch, it might stick
> > to say negative patches or loops on misfolded proteins. BTW, there are a
> > couple of putative Ni/Fe hydrogenases reported for E.coli that might also be
> > the source of contamination for IMAC.
> > Emir
>
> . . . there are a couple of papers about protein engineered to have some
> histidines in the middle of the primary sequence so they will bind Ni-NTA.
> I will have to think about where the refs are though.  I'll report back
> later!  Regards, Mike.

. . . Here is one.  But I am sure I have another somewhere:

Pfrepper et al 1997.  Biochemical and biophysical research communications
237:548-553.

Regards, Mike.




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