Question - UV crosslinking DNA

Bryan J. Maloney cavaggione at sbcglobal.net.spam.must.die.com
Mon Oct 21 22:37:10 EST 2002


erica.veysey at tufts.edu wrote in
news:20021009124100.4186.qmail at ww02.hostica.com: 

> I've seen some Southern Blot protocols that suggest crosslinking both
> sides of the membrane to immobilize the DNA.  What is the reason for
> this?  Is it necessary?  Thanks! 

It has been my experience that, when probing with a digoxygenin-labeled 
probe, crosslinking both sides of the blot significantly reduces 
background.



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