Electrocompetent cells

[Bryan J. Maloney]

dk at no.email.thankstospam.net (D.K.) wrote in
news:ap567g$qpv$1 at news.doit.wisc.edu: 

>  Philipp Wechner <philipp.wechner at uibk.ac.at> wrote:
>>Same protocol here. Use Cells from a culture between OD600= 0.5-0.8.
>>After the culture reachted 0.8 the cells are getting worse for
>>electroporation. 
> 
> All these numbers in all protocols are of dubuous value because 
> OD600 is spectrophotometer-dependent reading. What spec did
> you use? 

Moral:  It's a good idea to start by spending some time going between 
spectrophotometer and more direct cell counting.