Electrocompetent cells

Bryan J. Maloney cavaggione at sbcglobal.net.spam.must.die.com
Tue Oct 22 23:17:09 EST 2002


dk at no.email.thankstospam.net (D.K.) wrote in
news:ap567g$qpv$1 at news.doit.wisc.edu: 

>  Philipp Wechner <philipp.wechner at uibk.ac.at> wrote:
>>Same protocol here. Use Cells from a culture between OD600= 0.5-0.8.
>>After the culture reachted 0.8 the cells are getting worse for
>>electroporation. 
> 
> All these numbers in all protocols are of dubuous value because 
> OD600 is spectrophotometer-dependent reading. What spec did
> you use? 

Moral:  It's a good idea to start by spending some time going between 
spectrophotometer and more direct cell counting.




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