PI3K assay

Siddhartha S Mitra ssmitra at buffalo.edu
Thu Oct 24 18:51:16 EST 2002

I am trying to see PI3K activation in xenopus oocytes. can any one 
reccomend a good Ab for doing an IP against PI3K. Upstate Biotech has a 
Ab against the p85 subunit which they reccomend for the IP for a PI3K 
assay but the catalytic subunit is the p110. Is the p110 bound to the 
p85 subunit the active subunit?
Any suggestions would be welcome

Sid Mitra

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