Protein purification with intein tag

Joe Doe none at mail.utexas.edu
Wed Oct 30 20:14:37 EST 2002


In article <82fd6297.0210300225.68a0842 at posting.google.com>,
het_obf at rediffmail.com (OBF) wrote:

: does it work with all
> proteins? is the cleavage of the tag 100%? is the final eluted protein
> >95% pure (on the picture they show on the above website, the final
> protein doesn't look completely pure)?
> 
> Thanks in advance!
> Willem

We used it to purify lactococcus lactis LtrA protein.  In general it
worked well and had the added bonus of making this protein slightly more
soluble.  Cleavage is not a problem, expression levels were relatively low
for us (we used a very early version of the vector).  

Roland



More information about the Methods mailing list