Protein purification with intein tag
none at mail.utexas.edu
Wed Oct 30 20:14:37 EST 2002
In article <82fd6297.0210300225.68a0842 at posting.google.com>,
het_obf at rediffmail.com (OBF) wrote:
: does it work with all
> proteins? is the cleavage of the tag 100%? is the final eluted protein
> >95% pure (on the picture they show on the above website, the final
> protein doesn't look completely pure)?
> Thanks in advance!
We used it to purify lactococcus lactis LtrA protein. In general it
worked well and had the added bonus of making this protein slightly more
soluble. Cleavage is not a problem, expression levels were relatively low
for us (we used a very early version of the vector).
More information about the Methods