expression:82 kDa

Susanne rohrerspamsusannespam at hotmail.com
Thu Oct 31 04:16:45 EST 2002



Richard P. Grant wrote:

>In article <3DBF
>
>
>
>Richard P. Grant wrote:
>
>  
>
>>In article <3DBFF94D.786E4D9D at imb-jena.de>,
>> aidabaha at imb-jena.de (Aida Baharuddin) wrote:
>>
>>    
>>
>>>Is it possible to express a big protein,
>>>like in my case the size is 82 kDa?
>>>      
>>>
>>
>>'big'?
>>
>>Hah!  That's *tiny*!!
>>
>>
>>Ahem.  Yes, it is eminently possible.
>>
>>    
>>
> Hehe, poor "big game" biologists. Microbiologists seem to have it easier.
>
> try the pET system (from Novagen, you can probably borrow it from 
> someone) using BL21 DE3 cells - do yo want to tag it? the most 
> frequently used tag is obviously polyhistidine.
>
>  
>




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