expression:82 kDa
mfowle1 at lsuhsc.edu
mfowle1 at lsuhsc.edu
Thu Oct 31 21:06:42 EST 2002
Definitely possible...dare I say quite easy. Whether you express it bacterially or in a mammalian cell really depends on what you need it for. I routinely, and easily, express a 98 KDa protein in both. However, when I express a GST-tagged protein for pull down assays I have to grow lots in bacteria to get enough to use for the assay. But that isn't a big deal. Just express it however you need to. I do lots of transcription assays in mammalian cells, and size hasn't been a problem (even when tagged with GFP). I just have to make sure it has a NLS because that big of a protein doesn't get into the nucleus by diffusion!
Marcie
Aida Baharuddin wrote:
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> Dear readers,
> Is it possible to express a big protein,
> like in my case the size is 82 kDa? predicted to be
> located in the cytoplasm.
> The protein is from Legionella pneumophila.
> Which host cell would be the most appropriate,
> e.coli or mammalian cell expression vector?
> Thank you,
> aida
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> Dear readers,
> <p>Is it possible to express a big protein,
> <br>like in my case the size is 82 kDa? predicted to be
> <br> located in the cytoplasm.
> <br>The protein is from Legionella pneumophila.
> <br>Which host cell would be the most appropriate,
> <br>e.coli or mammalian cell expression vector?
> <p>Thank you,
> <br>aida
> <br>
> <br>
> <pre></pre>
> </html>
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