expression of GST-fused proteins

laflonia at tx.technion.ac.il laflonia at tx.technion.ac.il
Mon Sep 2 09:17:51 EST 2002


Hi

I did cloning of 3 proteins in the vector pGEX-4T. The problem is that I get small amounts of the fused proteins (detectable only in Western and not Coomassi). While GST alone ( control) shows a very good expression as detected by Coomassi.

I would appreciate any suggestion about improving expression of GST-fused proteins.

Flonia 

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