low RNA content despite high A260/280 Ratios

Thomas Mohr "thomasmohr[delete-for-correct-address]" at aon.at
Fri Sep 6 14:55:03 EST 2002


Dear colleagues,

recently we observed a high A460/A280 Ratio (>2.0) but a
formamide-gelelctrophoresis revealed very low contents of ribosomal RNA.
We did not observe signs of degradation (smears, etc).

The RNA was isolated by treatment with RNAzol, followed by an
Chloroform-Isopropanol-ethanol extraction.

Does anybody have an idea what might have gone wrong, resp. why this
high rations occured ?
Any response to thomas.mohr at utanet.at would be greatly appreciated.
Thanks,
Thomas



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