low RNA content despite high A260/280 Ratios
"thomasmohr[delete-for-correct-address]" at aon.at
Fri Sep 6 14:55:03 EST 2002
recently we observed a high A460/A280 Ratio (>2.0) but a
formamide-gelelctrophoresis revealed very low contents of ribosomal RNA.
We did not observe signs of degradation (smears, etc).
The RNA was isolated by treatment with RNAzol, followed by an
Does anybody have an idea what might have gone wrong, resp. why this
high rations occured ?
Any response to thomas.mohr at utanet.at would be greatly appreciated.
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