DOP-PCR high MW smearing

[Michael Witty]

On Mon, 9 Sep 2002, Ian Roberts wrote:

> Dear All,
>
> Recently I've been getting unusually large smears in my DOP-PCRs that go all
> the way back to the wells!  I've no real explanation for this observation,
> except some sort of contamination as it appears in the water control too.
> However, this is also sporadic.
>
> Has anybody else seen such smears?  If so I'd like some suggestions.  I've
> also heard that it might be something to do with low concentration of
> template.
>
> Regards,
> Ian

. . . is the template a circular plasmid?  Mike.