DOP-PCR high MW smearing
mw132 at mole.bio.cam.ac.uk
Mon Sep 9 10:58:03 EST 2002
On Mon, 9 Sep 2002, Ian Roberts wrote:
> Dear All,
> Recently I've been getting unusually large smears in my DOP-PCRs that go all
> the way back to the wells! I've no real explanation for this observation,
> except some sort of contamination as it appears in the water control too.
> However, this is also sporadic.
> Has anybody else seen such smears? If so I'd like some suggestions. I've
> also heard that it might be something to do with low concentration of
. . . is the template a circular plasmid? Mike.
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