DNA purification after agarase treatment

Peter Ianakiev ianakiev at genome.wi.mit.edu
Mon Sep 9 15:03:14 EST 2002


Hi Chris,

Electroelution in dialysis tubing seems to work great for PFGE fragments.

Cheers
Peter

C Coward wrote:

> Greetings,
>
> We're considering using beta-agarase to prepare HMW DNA from a pulse-field
> gel slice. We're wondering about the best method to purify the DNA after
> agarase treatment (the downstream application is a transposition reaction
> which we need pretty pure DNA for). Suggestions so far are
>
> -drop dialysis (any suggestions of membrane pore size?)
> -isopropanol precipitation, which apparently shouldn't precipitate the
> agarase digestion products (anyone with any experience of this?)
>
> any other suggestions?
>
> TIA
>
> Chris
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