DNA purification after agarase treatment

Peter Ianakiev ianakiev at genome.wi.mit.edu
Mon Sep 9 15:03:14 EST 2002

Hi Chris,

Electroelution in dialysis tubing seems to work great for PFGE fragments.


C Coward wrote:

> Greetings,
> We're considering using beta-agarase to prepare HMW DNA from a pulse-field
> gel slice. We're wondering about the best method to purify the DNA after
> agarase treatment (the downstream application is a transposition reaction
> which we need pretty pure DNA for). Suggestions so far are
> -drop dialysis (any suggestions of membrane pore size?)
> -isopropanol precipitation, which apparently shouldn't precipitate the
> agarase digestion products (anyone with any experience of this?)
> any other suggestions?
> Chris
-------------- next part --------------
A non-text attachment was scrubbed...
Name: ianakiev.vcf
Type: text/x-vcard
Size: 308 bytes
Desc: Card for Peter Ianakiev
Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/20020909/f6ac29d8/ianakiev.bin

More information about the Methods mailing list