Mixing vectors and cells

Joe Bagshaw jbagshaw at wpi.edu
Tue Sep 10 11:44:13 EST 2002


hi folks,

I have an interesting question for you all.  Does anybody think that using
Invitrogens pRSET A plasmid with Stratagene's BL21:DE3 cell line would
result in some unforeseen difficulty during induction?  On paper it doesn't
seem like this should make a differnece but I would like to know if anyone
has had any positive or negative experience mixing and matching seemingly
compatible products from different companies.

Thanks,
Alex Borrelli





More information about the Methods mailing list