Mixing vectors and cells

Philipp Wechner philipp.wechner at uibk.ac.at
Wed Sep 11 02:40:43 EST 2002


I am using Novagen´s pET-vectors in BL21....

Is there anything special with your plasmid? - if not, then there should not
be a problem.


Joe Bagshaw schrieb:

> hi folks,
>
> I have an interesting question for you all.  Does anybody think that using
> Invitrogens pRSET A plasmid with Stratagene's BL21:DE3 cell line would
> result in some unforeseen difficulty during induction?  On paper it doesn't
> seem like this should make a differnece but I would like to know if anyone
> has had any positive or negative experience mixing and matching seemingly
> compatible products from different companies.
>
> Thanks,
> Alex Borrelli




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