kirkwooc at cryptic.rch.unimelb.edu.au
Wed Sep 11 18:41:08 EST 2002
We are about to revisit MAb production after several years. We are
using the cell line Sp2/0 to fuse with the spleen cells we obtain
from mice immunized with our expressed protein.
Our old protocol called for both FBS and HAT to be used in the
initial fusion, then after positives cells were identified they were
weaned of HAT, with FBS still included in the media. I quess the
question is two parts
1) can serum free media be used in these fusions, instead of FBS?
2) if not can the positives be also weaned of FBS?
Does anyone have a protocol they could provide?
More information about the Methods