Methanol/tankblot buffer

Carmen Eckerich c.eckerich at
Thu Sep 12 06:38:33 EST 2002

Hi all !

We had an diskussion about the different protocols for western
bloting in our lab.
I found protocols for tank blotting buffer with and without methanol.
Does anybody know the funktion of the methanol in this buffer ?
Depends it on the membrane used (Nitrocellulose vs. PVDF) ?

Thanks in advance


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