Methanol/tankblot buffer

Philipp Wechner philipp.wechner at
Thu Sep 12 07:03:56 EST 2002

As far as I know the methanol avoids that the gel swells during

Carmen Eckerich schrieb:

> Hi all !
> We had an diskussion about the different protocols for western
> bloting in our lab.
> I found protocols for tank blotting buffer with and without methanol.
> Does anybody know the funktion of the methanol in this buffer ?
> Depends it on the membrane used (Nitrocellulose vs. PVDF) ?
> Thanks in advance
> Carmen

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