Methanol/tankblot buffer

[Philipp Wechner]

As far as I know the methanol avoids that the gel swells during
blotting.

Carmen Eckerich schrieb:

> Hi all !
>
> We had an diskussion about the different protocols for western
> bloting in our lab.
> I found protocols for tank blotting buffer with and without methanol.
> Does anybody know the funktion of the methanol in this buffer ?
> Depends it on the membrane used (Nitrocellulose vs. PVDF) ?
>
> Thanks in advance
>
> Carmen