Methanol/tankblot buffer

Philipp Wechner philipp.wechner at uibk.ac.at
Thu Sep 12 07:03:56 EST 2002


As far as I know the methanol avoids that the gel swells during
blotting.

Carmen Eckerich schrieb:

> Hi all !
>
> We had an diskussion about the different protocols for western
> bloting in our lab.
> I found protocols for tank blotting buffer with and without methanol.
> Does anybody know the funktion of the methanol in this buffer ?
> Depends it on the membrane used (Nitrocellulose vs. PVDF) ?
>
> Thanks in advance
>
> Carmen




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