How to get a sufficent DNA from gel for ligation?

Philipp Wechner philipp.wechner at uibk.ac.at
Fri Sep 13 03:11:00 EST 2002


Your "ligation product did not show enough growth for transformation"? What does this mean?

tevinxxx at hotmail.com schrieb:

> Every time, I cut DNA from the gel, but my ligation product did not show enough growth for transformation. Does anyone have better ideas for gel elution process and ligation?
>
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0
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