Cibacron blue ourification of ADH?

Fergus Doherty Fergus.Doherty at nottingham.ac.uk
Mon Sep 16 03:52:48 EST 2002


Has anyone experience of purification of yeast alcohol dehydrogenase using
the dye ligand Cibacron Blue F3GA?  I have tried it many times without
success.  Start/binding buffer 20mM Tris, 5mM mercaptoethanol, 5mM MgCl2,
0.4mM EDTA, as described in several protocols but ADH doesn't bind!  Plenty
of ADH activity.  Any help appreciated as lab class approaches!

-- 
Fergus Doherty,
School of Biomedical Sciences,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)



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