How to get a sufficent DNA from gel for ligation?

JonnyR9 jonnyr9 at hotmail.com
Mon Sep 16 12:34:51 EST 2002


I regularly use the APBiotech GFX gel extraction kit from gels made
and run with TAE buffer (Tris-acetate EDTA, look it up),

The yields are grrreat- but don't elute with TE, use hot water (60C)
instead.

If you are eluting DIG-labelled probes, add 1% SDS to your water,

Jon

tevinxxx at hotmail.com wrote in message news:<20020912184643.18044.qmail at ww02.hostica.com>...
> Every time, I cut DNA from the gel, but my ligation product did not show enough growth for transformation. Does anyone have better ideas for gel elution process and ligation?
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0



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