Cloning problem

[Emir Khatipov]

How do you know you ligation worked?
E.

"JSUPolek" <jsupolek at aol.com> wrote in message
news:20020916134325.00833.00004307 at mb-fe.aol.com...
> Does anyone know of reasons why DH5-alpha cells would be spitting out my
insert
> in pUC 19?  If I'm not mistaken, DH5-alpha lacks endonuclease activity, so
how
> is it managing to kick it out?  I know the remaining plasmid is kept
because it
> grows on subsequent Amp+ plates.  Thanks