Cloning problem

Duncan Clark junk at hgmp.mrc.ac.uk
Tue Sep 17 03:19:07 EST 2002


Historians believe that in newspost 
<20020916134325.00833.00004307 at mb-fe.aol.com> on Mon, 16 Sep 2002, 
JSUPolek <jsupolek at aol.com> penned the following literary masterpiece:
>Does anyone know of reasons why DH5-alpha cells would be spitting out my insert
>in pUC 19?  If I'm not mistaken, DH5-alpha lacks endonuclease activity, so how
>is it managing to kick it out?  I know the remaining plasmid is kept because it
>grows on subsequent Amp+ plates.  Thanks

Search the bionet archives. Umpteen possible reasons why a plasmid will 
re-arrange or delete an insert - regardless of it being in an endA 
E.coli.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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