gel shift mobility assay

Julia jul2 at pitt.edu
Wed Sep 18 07:48:16 EST 2002


Hi,
How do you determine EMSA specificity?
If unlabeled probe decreases the intensity of the bands,
does it mean that it is specific?
What if it doesn't?
What if we mutated the probe and it doesn't change the intensity of the
band anymore.
Thank you in advance,
Julia





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