cell arrestation in metafase

Goedele.Maertens at fys.kuleuven.ac.be Goedele.Maertens at fys.kuleuven.ac.be
Thu Sep 19 06:05:59 EST 2002


I arrested HeLa cells with nocodazole (0.8µg/mL) for 15 hrs and most of my 
cells were in a condensed chromatine stage.  Is it possible to wash nocodazole 
away and let the mitosis process go on (to get anafase cells) or do I have to 
use a lower concentration of nocodazole and/or longer incubation?
Any suggestions are welcome.

Kind regards,

Goedele Maertens

KULeuven
Laboratory of Biomolecular Dynamics
Belgium
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